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#Perform vs preform how to
#Perform vs preform software

The in silico PCR facilitates the computational analysis of our PCR reaction. If it binds to more than one location, it will give non-specific bands of the DNA, non-specific binding is the major limitation of any primer sequences. The primer must be specific to one target. One of the important properties of any primer is its specificity. For different PCR template DNA, primers are different. We have to design primers every time for every different PCR reaction. The prime is a very important component of the PCR reaction. For more detail on PCR primer please read our article: PCR primer design guidelines The primer must be 18 to 22 nucleotides long, having 50% of GC content and can bind only at the specific location to our DNA of interest. It provides the 3′ end to the Taq DNA polymerase to expand the growing DNA strand.

The primers are the short single-stranded DNA oligonucleotides used to amplify DNA in vitro. Now lets roughly understand about the primers. By doing this we can analyze the efficiency and the sensitivity of the primers.

#Perform vs preform software

The software also interprets the results computationally and gives a rough idea about the product size of the amplicon. It gives the idea of at which position the primer going to binds, whether it is bind to the exact target location or any other non-specific location. It actually, examines the primer set that we selected against the target sequence or genome. Here, the software checks the primers (what we designed) against the genome of our interest. In silico PCR is a computer application based analysis of our PCR reaction with the help of the primer information.

#Perform vs preform how to

In this article, we are giving you a comprehensive guide on how to do in silico PCR.įirst of all, I will explain to you in silico PCR in a simple language, However, the most popular software is ePCR and in silico PCR by the UCSC genome browser. “Any process done on the computer-based application is called in silico process.”ĭifferent types of in silico PCR software are available online. Wikipedia defines the term in silico as “ perform on a computer or via computer simulation. Read our interesting article on PCR: A Complete Guide of the Polymerase Chain Reaction.

What is a primer and what type of primers are used in the PCR?.

To understand the concept of the in silico PCR, we have to answer this question,įor that, we have to first understand the meaning of the in silico and Instead, only sequence information and primer data is needed to do analysis. Thus if it is not good, it saves time, chemicals, buffer and man-power.Īctually it is nota true PCR reaction, thus dNTPs, primers, buffer and DNA are not used in it. The objective of performing this type of computational analysis is to predict the result of actual PCR reaction.īut why to do in silico PCR? because, we can get some rough idea of how our reaction behaves during the actual PCR. Here in the in silico PCR, we are performing a PCR reaction virtually, on computer software, therefore it is also known as electronic PCR or virtual PCR. “An in silico PCR is a computational tool used to determine the results of practical PCR reaction computationally using a set of the selected primers.”